L> gartenbau 202h lab 4horticure 202h lab 4 insulating pigmentation of plants introduces the physical appearance of plants that can deceive what can be found in our eyes, reaction in the wavelength of their wavelength Light reflects the surface of the tree. The function of different pigments in plant cells is these pigments in the patches (chloro, chromoplasts, amyloplast, etc.) and / or do not concentrate and absorb some length of the wavelength (and Therefore, energy) the wide polarization of chlorophyll and carotenoids, very insoluble (hydrophobic or non -polar) and is integrated into the film with anthocyanas, very soluble in water. The solvents are used with good chromatography of very soluble homosexual polarization.
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. This is mixed according to the ratio of changes in other polarized solvents, such as experimental water background: at 80% acetone is often used to extract chlorophyll from plant samples, and because it contains 20 % WCT TLC of water and chromatography in the system chromatography: Aceton: acetone: aceton: (60:40 V / v) of the three mit different culo solution (1- Propanol: acetone: water) allows separation of three Birthday (CH Lorofilos, Carotinoids, Antocianasase Anasas). ) The inequality is Acetone in all its coms must not be changed with 10% of the solvents of V / V: Acetone (60:40 V / V).
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By 10:10:80 will be done by groups working from three groups, a student of each group can perform 075 ml of each solvent in a test pipe and labeled with the corresponding amount of solvent, Prepare the remaining two solvents for students extracted from the pigments of 3 sources, two sources, sweet potatoes "Margarita" Presentation of micro tubes in equilibrium and centrifugal position for 3 minutes with the maximum speed of the TLC bands before cutting. With the upper part with the upper part with the number of solvents and with the help of micropipeta, remove a total of about 6 to 10 in its example in about 1 cm of Aliqutes from the bottom of the strip (its location is no. direct contact with solvent in the tube)) Wait between each part 2 to make the sample dry before adding more.
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Of course, the object includes the surface of the dry TLC strip before placing its TLC bands into the tube and developing chromatography. This may take 10 to 30 minutes immediately, but this may take time. , it can be visually seen after development, removing TLC bands from the tubes and quickly and quickly and quickly the FrONT solvent, origin, pigments, finally dry chromatography Under the ultraviolet light of the laboratory on February 17, 2003.
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Click how to expand the click image to expand the click to expand the analysis: 1 Save your result by drawing and labeling for Your Schnomatogram chromatographers have been better described for the data stated in which the system works better to work to separate the three pigments? Why? 2 Explain what you have observed when you have exposed its UV chromatography, and why does it appear? 3 Observe the absorption spectrum of 3 extracts of different plants received with scanning spectroscopy